Can anyone point me to a document describing quality control standards for NGS generally and for the SOLiD 5500 specifically? I understand the BAMStats module offers quality control metrics but I need documentation to interpret them.
The current project is RNA-seq. I have seen references for third party tools like RNA-SeQC and RSeQC. Are these applicable to SOLiD data or tuned for Illumina? These tools describe a number of metrics but they do not describe how these metrics should be interpreted (i.e. what values indicate quality data? what values indicate questionable data).
Thanks,
Mike
The current project is RNA-seq. I have seen references for third party tools like RNA-SeQC and RSeQC. Are these applicable to SOLiD data or tuned for Illumina? These tools describe a number of metrics but they do not describe how these metrics should be interpreted (i.e. what values indicate quality data? what values indicate questionable data).
Thanks,
Mike
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