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Do you have the protocol for setting the Z focal range? this is a procedure we carry out before each run? I'm not sure if I can attach it here so I'll send it to you as a private message -
Focus Range setting with 1.2.1 ICS software
Hi everybody
Yesterday we would to use SOLiD5500 for first time after software (ICS 1.2.1) update, but we couldn't see the deposited bead on Imager UI
Anybody have a similar experience ????
What can I do to set correctly the Focus Range???
I attached a screenshot of problem
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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