Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • JNK
    started a topic SOLiD 5500xl mate pair lib starting questions

    SOLiD 5500xl mate pair lib starting questions

    hi,
    I have just started to work on solid 5500xl and planning to prepare a mate pair library 3kb.
    I just need to know what would be the imp steps to be taken care during library preparation step.
    The nick translation time given in the protocol is 10-11 mins. Since the nick translation time decides the length of the final library, what time of incubation should i keep (ie 10 or 11 mins.) Right now I am following the protocol as it is. I would also like to know the range of the final library, with the peak, as assessed on Bioanalyzer
    Anybody tried increasing the nick translation time and seen the results.

    Also what would be the maximum no. of amplifications that can be given.

    Thanks.

Latest Articles

Collapse

  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    06-06-2024, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 06:54 AM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-14-2024, 07:24 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-13-2024, 08:58 AM
0 responses
15 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-12-2024, 02:20 PM
0 responses
17 views
0 likes
Last Post seqadmin  
Working...
X