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  • SLCoon
    Junior Member
    • Apr 2014
    • 1

    ribosomal profiling on a SOLiD 5500?

    I'm starting a ribosomal profiling experiment where I want to do the sequencing on a SOLiD 5500xl. I've done some work with Illumina sequencing. Is there a protocol for making the libraries for the SOLiD? I think I understand that I need to use specific DNA/RNA adapters (Adapters A or B), but these do not seem to currently commercially available. Do i need to go ahead and synthesize them myself? Has anyone done this?
    Thanks, SLCoon

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  • SEQadmin2
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    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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