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.baf from BFAST alignment output to .BED format? Thanks.
J
.baf from BFAST alignment output to .BED format? Thanks.
J
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There are many ways. You can convert the BAF file to the SAM/BAM format using the "bfast postprocess" option, which you should use anyways to prioritize alignments. Then you can write your own program or check out this thread to convert SAM/BAM to BED.Originally posted by JohnK View Post -
I think it's time for a giant table of file formats..."I have" vs "I want"....Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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