I've heard that trimming is not as efficient as alignment in the pipeline and then building up or down from there.
-
-
Could you elaborate? What do you mean by 'efficient'?Comment
-
i think the small rna pipeline will use the adapter sequence to more efficiently trim reads. It's better to run them as 35bp than to trim them and try and map.Comment
-
cutadapt and most trimmers I know do use the adapter sequence to trim the reads (they align the adapter sequence to the end of the read). The alternative is to unconditionally remove the last x bases (or colors) of each read. I do not consider that to be adapter removal, it is way too inaccurate.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment