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chainTerminator

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Join Date: Jul 2010

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Big Dye Terminator

07-29-2010, 10:52 AM

Hello All-

Does anybody know how to make their Ready reaction mix and 5x Sequencing buffer? We've been trying to reverse engineer it but no luck. We use ABI's v3.1. Its pretty costly so we're trying to make our own! Any ideas?
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Nine Things a Sample Prep Scientist Thinks About Before Sequencing

by SEQadmin2

I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

Here are nine questions we think about, in roughly the order they matter, before...
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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