In the XXXX_.QV.qual file, there is a quality value for each position.
I wonder how they calculate this value and what is the meaning of this value.
For traditional sequecing, I think quality value is the P value of the corret read over the noise, then -10logP. However, for SOLID, every read is from 2 base pair, how do they get the quality value? Anybody can provide some advice or reference?
Thanks a lot!
I wonder how they calculate this value and what is the meaning of this value.
For traditional sequecing, I think quality value is the P value of the corret read over the noise, then -10logP. However, for SOLID, every read is from 2 base pair, how do they get the quality value? Anybody can provide some advice or reference?
Thanks a lot!
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