Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • SOLiDance
    Member
    • Jun 2010
    • 27
    #1

    Sth about RNA Barcode library

    Hello everyone~
    I am gonna prep several RNA Barcode libraries(fragment), The protocol is simple and almost the same as the normal RNA libraries(fragment).
    It's said the IA sequence will anneal with the one end of adaptors.(see the attachment) Then I compared the sequence of P1,P2 adaptors with the IA, couldn't find consensus seq.
    So I think maybe the adaptors Mix in RNA library is totally diff from DNA library? but in the Beads prep step,the primers are the same~ Maybe it's kind of silly question, But I really can't get a clue~Hope anyone give a hand
    THANKS A Lot!!
    Attached Files
    Tags: None
Previous template Next

Latest Articles

Collapse
  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
View All

ad_right_rmr

Collapse

News

Collapse
Topics Statistics Last Post
High-Resolution Sequencing Exposes Hidden Toxoplasma Diversity by SEQadmin2
Started by SEQadmin2, 07-02-2026, 11:08 AM
0 responses
14 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
07-02-2026, 11:08 AM  
New AI Model Captures Long-Range Genomic Signals to Improve RNA Splice Site Prediction by SEQadmin2
Started by SEQadmin2, 06-30-2026, 05:37 AM
0 responses
15 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
06-30-2026, 05:37 AM  
Large-Scale Protein Screen Uncovers Hidden Regulators of Alternative Polyadenylation by SEQadmin2
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
20 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
06-26-2026, 11:10 AM  
Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
54 views
0 reactions
 Last Post SEQadmin2
by SEQadmin2
06-17-2026, 06:09 AM  
View All
Working...