Hello everyone~I am gonna prep several RNA Barcode libraries(fragment), The protocol is simple and almost the same as the normal RNA libraries(fragment).
It's said the IA sequence will anneal with the one end of adaptors.(see the attachment) Then I compared the sequence of P1,P2 adaptors with the IA, couldn't find consensus seq.
So I think maybe the adaptors Mix in RNA library is totally diff from DNA library? but in the Beads prep step,the primers are the same~ Maybe it's kind of silly question, But I really can't get a clue~Hope anyone give a hand
THANKS A Lot!!
