Ovation RNA-Seq kit with SOLiD?

[phiS]

We're looking for a solution for doing RNA-Seq from precious samples (Trizol-extracted, silica-column-cleaned total RNA from plant material) where less than 1 µg of total RNA is available and there is no chance of ever getting more material. 1 µg total RNA is well below most of the recommendations for library preparations we've seen.

Do you have any suggestions regarding protocols or kits that have worked well before with low amounts of input RNA?

In particular, does anyone have experience with NuGen's Ovation RNA-Seq kit for RNA-Seq with SOLiD 4? Are there any pitfalls? Is there anything to look out for specifically? Do the resulting data look good (any bias?) or are there any problems?

[Erika Feltrin]

Ovation RNA-seq kit with SOLiD

Did you finally used the Ovation kit with SOLiD?
We are thinking to use it with our SOLiD 5500 machine but do not have any experience about this kit.

Best Regards
Erika

[phiS]

We came to the conclusion that it would likely work, but we didn't go ahead for unrelated reasons, i.e. we never tried it.