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  • genlyai
    replied
    Originally posted by GW_OK View Post
    Colleagues attending ASHG asked some questions for me. Here's the answers they got:
    1) How many pores/average read lengths/flowcell lifespan hours can one expect in the early access Minions?
    No info on pore number, they are no reporting it. Read length 5-15kb, lifespan 6-8 hrs. Accuracy depends on speed of reading.

    2) What's the shelf life of a Minion flow cell?
    Could not report to me a shelf life.

    3) Can Minions be used for multiple samples like a Gridion cassette?
    Single use only.
    I visited their booth and got slightly different info for point 1:

    - lifespan 20hrs
    - expect 400 functioning pores per minion cell
    - speed/accuracy is indeed dialable, with speeds between 10bp/sec and 100bp/sec

    Again, this is all by recollection alone (so may be wrong), but I thought it might be of interest. Is anyone in the early access program?

    Leave a comment:


  • GW_OK
    replied
    Go read this about library preps and some nice pics of the minion.

    Leave a comment:


  • GW_OK
    replied
    Originally posted by GenoMax View Post
    It would make sense if the USB device (MinIon) was $1K and the "flowcells" were priced separately, dare we expect "reasonably".
    I have a feeling this is going to be a razor blade marketing strategy. They mention the "flow cells" as being $999. I'll bet you get the Minion free with your first purchase, then you pay for the $1k flowcell every time you want to run it.

    Actually, on even further reading, I bet you pay $1k refundable deposit on the device, then $1k for every run on said device, and you can get your deposit back on return of the device.

    Of course this is all speculation at the moment and based solely on interpreting the press release of a very secretive company.

    Leave a comment:


  • rahulvrane
    replied
    Thanks a lot for that GW_OK!

    I guess the only way the minions are winning is if they can extend the lifespan of the flowcells.

    Leave a comment:


  • GW_OK
    replied
    Colleagues attending ASHG asked some questions for me. Here's the answers they got:
    1) How many pores/average read lengths/flowcell lifespan hours can one expect in the early access Minions?
    No info on pore number, they are no reporting it. Read length 5-15kb, lifespan 6-8 hrs. Accuracy depends on speed of reading.

    2) What's the shelf life of a Minion flow cell?
    Could not report to me a shelf life.

    3) Can Minions be used for multiple samples like a Gridion cassette?
    Single use only.

    Leave a comment:


  • GenoMax
    replied
    Originally posted by GW_OK View Post
    At the beginning of the article they mention the "flow cells" and "usb device" as being separate entities. Also, on the ONT website, they say you can dry down the flowcells before you send them back, a condition of the early access program. This, to me, seems as though the MinIon can accept single use cartridges.
    Ah this may is the part that has taken them long to optimize.

    It would make sense if the USB device (MinIon) was $1K and the "flowcells" were priced separately, dare we expect "reasonably".

    Leave a comment:


  • GW_OK
    replied
    Originally posted by GenoMax View Post
    You have to send the MinIons in for recycle/reuse, if I remember correctly.
    At the beginning of the article they mention the "flow cells" and "usb device" as being separate entities. Also, on the ONT website, they say you can dry down the flowcells before you send them back, a condition of the early access program. This, to me, seems as though the MinIon can accept single use cartridges.

    Leave a comment:


  • rahulvrane
    replied
    Maybe I am a bit new to the whole ONT discussion.. But from my read of the website, I see they talk about a 'Run till you get sufficient data' workflow. (Kinda enticing )

    But has there been any mention of just how long each of these can be run and what the final pore density would be per chip for GridION and MinION?

    Obviously the GridION would be a legit superior, but just how far behind is the MinION in performance (or throughput)? ... Any clues I might have missed so far or new ones from the latest ASHG?

    Cheers!

    Leave a comment:


  • GenoMax
    replied
    Originally posted by GW_OK View Post
    The MinIons also appear to be re-usable! So, so cool.
    You have to send the MinIons in for recycle/reuse, if I remember correctly.

    The article you linked says "participants can order additional flow cells for $999 a piece". I hope that is just during the trial and that the final cost will not be that high.

    It would be difficult to keep tens on hand if they are going to cost $1K each.

    Leave a comment:


  • GW_OK
    replied
    ONT is the Kool-aid Man busting through the wall:
    "OH YEAH!"

    PacBio shares already down 15%.
    Some extra info at the bottom of the GenomeWeb article

    The MinIons also appear to be re-usable! So, so cool.

    Leave a comment:


  • Fred
    replied
    Hi,

    Why not try it ?

    https://www.nanoporetech.com/technol...cess-programme

    Fred

    Leave a comment:


  • DaleYuzuki
    replied
    Oxford Nanopore at ASHG

    Here at the ASHG tradeshow floor setup, was able to spot the Oxford booth. (Attached is a photo, and you can spot the stack of GridIONs on top of the table there.)

    I heard a rumor yesterday about them but won't engage in any rumor-mongering.
    Attached Files

    Leave a comment:


  • GW_OK
    replied
    ONT put up some new movies in the past 4 days. New looks at the GridION cartridge as well as miRNA sequencing and protein detection.

    Leave a comment:


  • gringer
    replied
    Apparently position 654 in the exhibition centre at ASHG 2013:

    http://www.ashg.org/cgi-bin/2013/ashg13fp.pl

    [top left]

    Leave a comment:


  • BBoy
    replied
    Originally posted by NextGenSeq View Post
    You could check their website.

    https://www.nanoporetech.com/news/events
    ASHG 2013. Oxford Nanopore will be exhibiting at ASHG 2013 in Boston, 22-26 October. http://www.ashg.org/2013meeting/
    Hopefully more than mockups with plexiglass cutouts like last time around? Unless they show something this will start turning into the mother of all vaporware. Given that there are no leaks (and there were leaks before AGBT 2012) I am not too hopeful. Will be glad to eat my words.

    Leave a comment:

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