New NEXTflex DNAseq Library Prep Kits and Barcodes

[Bioo Scientific]

Bioo Scientific launched the NEXTflex™ DNA Sequencing Kits and NEXTflex™ DNA Barcodes, designed to provide flexibility and high-throughput capabilities in sequencing applications at a cost savings. The NEXTflex DNA Sequencing Kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. By making changes to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters and expect to see better binding efficiencies. The NEXTflex DNA Sequencing Kits are designed to prepare single, paired-end and multiplexed genomic DNA libraries for sequencing using Illumina/Solexa GAII and Hiseq 2000/1000 platforms.

The enhanced NEXTflex DNA Sequencing Kits simplify workflow by using master mixed reagents and magnetic bead-based cleanup, reducing pipetting and eliminating several time-consuming steps in library preparation. In combination with the NEXTflex DNA Barcode Kits, the NEXTflex DNA Sequencing Kits can be used to profile and study up to 48 samples simultaneously. The NEXTflex DNA Barcodes are adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. This automation-friendly format enables multiplexing of up to 48 samples for a total of 384 reactions. The NEXTflex DNA Barcodes are available in sets of six, 12, 24 and 48 unique adapters. The ability to pool samples in an efficient way significantly decreases hands-on time, while providing robust data quality.

[gary]

NEXTflex™ DNA Sequencing Kits & AIR™ DNA Barcodes

Hi:
Can I use the adaptors and primers in AIR™ DNA Barcodes Kit with NEXTflex™ DNA Sequencing Kits to prepare a paired-end library?

Thanks!

[Bioo Scientific]

Hi Gary,

Yes, the adapters and primers in the AIR™ DNA Barcode Kit can be used to prepare paired end libraries with the NEXTflex™ DNA Sequencing Kit. The adapters included in the NEXTflex DNA Seq Kit and the NEXTflex™ DNA Barcode Kits can also be used to make paired end libraries. Like the AIR DNA Barcodes, the NEXTflex DNA Barcodes include up to 48 different barcodes which can index up to 48 samples. The difference between AIR and NEXTflex barcodes is how the barcode is actually incorporated. In the AIR Barcodes it is added during PCR as a third barcoded primer. In NEXTflex Barcodes it is added right at the adapter ligation step.

[Efortier]

Hi,

Is the NEXTflex Barcode kit (set of 48) compatible with the TruSeq library kit from Illumina?