13 results in 0.0023 seconds.
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Why is my DNA concentration and absorbance ratios fluctuating?
I have done DNA extraction on human saliva using Promega ReliaPrep™ Blood gDNA Miniprep System that have been expired for more than five years now.... -
bioanalyzer and qubit disagreement
We prepare clinical samples with a formal SOP and do not regularly see irregularities. Today we have a capture pool of samples that quanted at 24.0ng/uL... -
qubit standards left at RT overnight
Are the standards okay after having been left out overnight at RT?
Forgot to put them away--Just curious if they are still usable or if... -
Quantitation of Nextera XT library
I have searched the forum without success so apologies in advance if I have missed this elsewhere.
We are sequencing pooled Nextera XT... -
Is Qubit good for multiple samples?
Hello,
We've been having some quantitation issues recently, I'm wondering whether the Qubit fluorometers are good for processing lots... -
qubit and picogreen discrepancies
We're a small lab that has done most of our quantitation using nanodrop and qubit, depending on the application. However, as we try to prepare multiplexed... -
Quantitation Variations with Qubit
Hello All,
I have run into quite a strange issue recently.
I have successfully and consistently been running 16S and 18S... -
Agilent TapeStation vs. Qubit: RNA concentration.
SCENARIO:
After RNA extraction (RNA-seq is my aim), I check the concentration of the extracted RNA with Qubit, and then run the extracted... -
qPCR vs Qubit, big difference
We have prepared a TruSeq DNA PCR-free library, and quantification with Qubit gives roughly 10 times higher concentrations than qPCR (range 4-50 times... -
Qubit - wrong values due to GFP?
Hi,
I performed a Qubit measurement on genomic DNA (BR) and took 2.5 µg according to the measurement for a reaction with a GFP-tagged... -
Qubit Quantification
Hi All,
I've been using Qubit dsDNA kits to quantify my DNA preps for about 12 months.
Recently we've had issues with low... -
Qubit vs Nanodrop discrepancies??
Hi all,
What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?
Our core facility has generated... -
Library Quantification Confusion!
I have just finished making some Illumina mRNA libraries using the v2 TruSeq Kit. I used the QUBIT to quantify samples, and got high concentrations (200-600ng/uL)....
