I'm planning to do a pseudohaplotype assembly with Hifiasm on PacBio HiFi wgs data for an XY male specimen. Does anyone know how Hifiasm will handle the sex chromosomes? I mean, will it give me primary and alternate assemblies for the sex chromosomes? How will it handle the pseudoautosomal regions of homology between the X & Y? Is there a strategy I should be using to limit the mess I make of the sex chromosomes? I'm new to all of this, so pretend I'm 8 years old.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM