Hi,
My goal is to obtain the sequence of a gene from several species. I know the sequence only in 2 species separated by ~30mya. Designing optimal primers is not an option, since if the PCR reaction doesn't work in one species, I wouldn't know whether its because the primers failed or the gene is absent. Southern is too cumbersome, in my opinion. Wonder if there is another way to do my job? I checked for commercial kits but most kits for targeted sequencing are in humans and I'm working in a non-model plant lineage.
I want to stress that I only want to get only selected loci (5 genes in my case)...
My goal is to obtain the sequence of a gene from several species. I know the sequence only in 2 species separated by ~30mya. Designing optimal primers is not an option, since if the PCR reaction doesn't work in one species, I wouldn't know whether its because the primers failed or the gene is absent. Southern is too cumbersome, in my opinion. Wonder if there is another way to do my job? I checked for commercial kits but most kits for targeted sequencing are in humans and I'm working in a non-model plant lineage.
I want to stress that I only want to get only selected loci (5 genes in my case)...
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