I use FACS sorted leukocytes to carry out ChIP-seq. After crosslinking in formaldehyde the cells become very sticky and difficult to pellet. Instead, they appear to adhere to the Eppendorf tube walls. I seem to loose a lot of cells at this stage. Any ideas how to prevent or overcome the problem?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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