I use FACS sorted leukocytes to carry out ChIP-seq. After crosslinking in formaldehyde the cells become very sticky and difficult to pellet. Instead, they appear to adhere to the Eppendorf tube walls. I seem to loose a lot of cells at this stage. Any ideas how to prevent or overcome the problem?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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