epiGBS

[Niels Wagemaker]

Nature methods epiGBS article:

Please notice our Nature methods epiGBS article on epiGBS:
epiGBS: reference-free reduced representation bisulfite sequencing
doi:10.1038/nmeth.3763

I made this thread to help get you information about new updates on the protocols (new enzyme combinations, adapter innovations) and to get in contact for help or collaborations.

Our great protocol will really help you to steam up your DNA genotyping and methylation research on big amount of samples for affordable costs.

Niels Wagemaker

[ecSeq Bioinformatics]

DNA Methylation Data Analysis Workshop
How to use bisulfite-treated sequencing to study DNA methylation

When?
22. - 25. November 2016

Where?
Leipzig, Germany

Link?

http://www.ecseq.com/workshops/workshop_2016-07-NGS-DNA-Methylation-Data-Analysis

[Niels Wagemaker]

Epigbs enzyme combinations

Next to the published enzyme combinations we have available:

Improved solutions for

Smal genomes: (arabidopsis): csp6I * NsiI
Average genomes: (scabiosa columbaria): AseI * NsiI
Large genomes: (sequoia): PacI *NsiI

All with methylation insensitive enzymen.
PacI needs different adapter set as other 2 enzyme combi's

More info: mail me