Hi! I have samples sequenced with NextSeq for the experiment protocol setup. We tested different kits (Nextera, Kapa, Qiagen) and apart from the donor samples, we sequenced ATCC bacterial mix with the addition of human cells, as well as ATCC genomic DNA. What I observe in QC reports is first of all, per base sequence content bias, but also disturbing GC content distributions for ATCC samples (yellow ones are the bacterial mix, the red one is genomic DNA). Would that suggest contamination or some other issue, or could it be a characteristic of those samples?
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM