Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • chansamuel
    Junior Member
    • Mar 2022
    • 1

    #1

    Metagenomic Taxonomic Profiling (beginner)

    Hello, a little bit about myself. I'm a first year bioinformatic grad student who somehow got himself into a mock metagenomic project (there will be an actual project in half a year). I have no prior experience with metagenomics side and there are no courses being offered in my university that covers the basic of metagenomic.

    For the project, I am expected to 1) find and download human metagenomic data (raw reads), 2) perform taxonomic assignment (taxonomic profiling, etc), and 3) perform comparative metagenomic is possible.

    I somehow managed to download files (in fastq.gz format) from the human microbiome project database. I vaguely know I should use software such as Kraken but I honestly have no clue (and no one to ask in rl) who should I even begin. If anyone can point me towards the right direction or leave hints, it will be a life saver.

Latest Articles

Collapse

  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2




    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
    Yesterday, 06:46 AM
  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
25 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-28-2026, 09:51 AM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
71 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...