Tweet
Pippin Prep for RNA?
For those of you familiar with the Pippin Prep, currently used for automated DNA size selection and band capture, your thoughts on whether or not Sage Science should commercialize a series of cassettes for RNA too? What RNA applications are of greatest interest? Thanks for your thoughts and help!
-
-
If these were denaturing gels that would work on strand denatured DNA as well, I think they would be very useful.
Subtext of a recent thread
is that short ssDNA/adapters may be annealing to longer library products -- preventing their removal via size-selection on dsDNA products. If the size selection were done on ssDNA there is no where for these short contaminants to "hide" and they should be removed.
--
Phillip -
Phillip - We can manufacture any standard precast gel chemistry, including denaturing gels that should enable this. Thanks for the link and the idea. I will discuss it with our team.
GaryComment
-
we would be interested in RNA cassettesComment
-
-
Seems like you have a ready-made list of beta testers right here in this very thread!
Comment
-
I agree with pmiguel. We see effects similar to the ones in the cited thread, and I think a denaturing gel to size select ssDNA fragments would be very helpful.Comment
-
RNA on Pippin Prep
Sounds good everyone. I will discuss this application with the team and Sage. Thanks for your thoughts, interest and willingness to test RNA cassettes. - GaryComment
-
Yes, send them to Purdue and we will test them on our Pippin prep.
I think there are lots of otherwise perfectly good libraries that are getting hosed by small "hitchhiker" amplicons that can't be removed with double-stranded size-selection methods. Maddening!
Well, there is always normal denaturing PAGE. But it always seems like such a hassle to extract the sample from polyacrylamide gels.
--
PhillipComment
-
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM -
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...07-09-2026, 11:10 AM -
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...07-08-2026, 05:17 AM
Comment