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  • HGENETIC
    still a novice
    • Jul 2010
    • 34
    #1

    phosphorothioate bond

    Hi, does anyone have experience of using blocking primers during a hybridisation step without including a phosphorothioate bond? I ordered some primers for use with TruSeq but forgot to add this bond when I sent the details to the primer synthesis company and I'm not sure if I should re-order them with the bond?

    Thanks

    H
    Tags: None
    • yoon
      Junior Member
      • May 2011
      • 6
      #2
      is this for exome capture protocol?

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        • HGENETIC
          still a novice
          • Jul 2010
          • 34
          #3
          Originally posted by yoon View Post
          is this for exome capture protocol?
          It's for a targeted capture protocol using an NimbleGen probe library so basically it's very similar.
          Last edited by HGENETIC; 10-05-2011, 05:41 AM.

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