Hi All,
I am facing lot of variations in my library quantitation using Qubit dsDNA BR assay kit. Lambda DNA stock concentration which is provided by Sigma has a documented concentration of 400ng/ul and Qubit shows above 550ng/ul. What might be the root cause? Is it pipetting error or people tend to get variations from sample to sample?
Please advise!
I am facing lot of variations in my library quantitation using Qubit dsDNA BR assay kit. Lambda DNA stock concentration which is provided by Sigma has a documented concentration of 400ng/ul and Qubit shows above 550ng/ul. What might be the root cause? Is it pipetting error or people tend to get variations from sample to sample?
Please advise!
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