Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Time
  • Show
Clear All
new posts
Previous template Next
  • prepagam
    Junior Member
    • Mar 2012
    • 7
    #1

    Bioruptor - DNA in TE?

    Various protocols for bioruptor shearing suggest that you reconstitute your DNA to your desired concentration in TE (e.g. 1ug DNA in 100ul TE). My DNA samples were eluted in Qiagen's AE elution buffer, and I wonder if I can shear in AE? I have little DNA for my samples (200-400ng), so I wasn't planning an ethanol purification step to concentrate them down, rather just use the eluted DNA in AE directly. Is this likely to be a problem?

    I'm preparing DNA for library prep for Illumina paired end sequencing - using Biooscientific barcodes and library prep kit.
    Tags: bioruptor, dna library prep, dna shearing
    • caesar1970
      Junior Member
      • Apr 2012
      • 1
      #2
      Yes, you can shear in AE buffer. It is very similar to TE buffer and should not be a problem at all.

        Comment

        • NextGenSeq
          Senior Member
          • Apr 2009
          • 482
          #3
          It's TE without the E (EDTA)

            Comment

            Previous template Next

            Latest Articles

            Collapse
            • SEQadmin2
              Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
              by SEQadmin2



              Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

              Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating              ...
              Yesterday, 09:55 AM
            • SEQadmin2
              New Genomics Technologies Take Aim at Long-Standing Limits
              by SEQadmin2


              Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

              We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing              ...
              09-28-2026, 10:25 AM
            View All

            ad_right_rmr

            Collapse

            News

            Collapse
            Topics Statistics Last Post
            Study Finds Chromatin Motion Holds Steady Until Cells Face Stress by SEQadmin2
            Started by SEQadmin2, Yesterday, 05:39 AM
            0 responses
            13 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            Yesterday, 05:39 AM  
            Larger Datasets Reveal Additional Switches That Control Gene Expression by SEQadmin2
            Started by SEQadmin2, 10-13-2026, 09:56 AM
            0 responses
            15 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-13-2026, 09:56 AM  
            Computational Tool Captures How Chromatin Folds at Scale by SEQadmin2
            Started by SEQadmin2, 10-09-2026, 11:14 AM
            0 responses
            27 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            10-09-2026, 11:14 AM  
            Spatial Transcriptomics and Metabolite Mapping Combined on a Single Tissue Slide by SEQadmin2
            Started by SEQadmin2, 09-29-2026, 09:51 AM
            0 responses
            78 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            09-29-2026, 09:51 AM  
            View All
            Working...