Illumina adapter--what can I dispense with?

[uberfinch]

Hi folks,


For my project I want to alter the 5' illumina adapter sequence (aka "universal" adapter) to make the 3'-most 11 bases different from the 11 bases on the other side (the symmetry in the two adapters screws up my experiment). Assuming I add a custom primer for read #1, does anyone expect this to be a problem?


Thanks!

[Cofactor Genomics]

Dear Uberfinch,
This should not be a problem, unless you will be running paired-end reads. You are unable to use a custom seq.primer on read two since each lane is not addressed separately at that stage.

Best of luck,

Jon