Hi folks,
For my project I want to alter the 5' illumina adapter sequence (aka "universal" adapter) to make the 3'-most 11 bases different from the 11 bases on the other side (the symmetry in the two adapters screws up my experiment). Assuming I add a custom primer for read #1, does anyone expect this to be a problem?
Thanks!
For my project I want to alter the 5' illumina adapter sequence (aka "universal" adapter) to make the 3'-most 11 bases different from the 11 bases on the other side (the symmetry in the two adapters screws up my experiment). Assuming I add a custom primer for read #1, does anyone expect this to be a problem?
Thanks!
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