Hi,
I have a bunch of 1-1.5 kb amplicons that I'd like to run on Illumina. Has anyone had success nebulizing such small fragments? Or perhaps using DNAse or restriction digestion to bring them down in size a bit? Primer design is tricky for my particular targets so re-designing to get optimal size fragments is not always possible.
I have a bunch of 1-1.5 kb amplicons that I'd like to run on Illumina. Has anyone had success nebulizing such small fragments? Or perhaps using DNAse or restriction digestion to bring them down in size a bit? Primer design is tricky for my particular targets so re-designing to get optimal size fragments is not always possible.
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