Hi guys,
I am happy to read your valuable discussion here. We recently had problem that the color balance of the sequencing is completely off, which caused majority of image pannel failed. On the WFA, every parameter looks fine except P2_Gain is too low (lower than 8). Is that color balance off is caused by the concatamerization of adaptors? We did not know this trick and did not dilute the adaptors while our ChIP DNA concentration is very low. If the P2_gain is a result of adaptor concatamerization, we can use it to titrate the adaptor dilution based on the concentration of ChIP Seq samples from our customers.
Thanks in advance
Thanks in advance.
Smallcreek
I am happy to read your valuable discussion here. We recently had problem that the color balance of the sequencing is completely off, which caused majority of image pannel failed. On the WFA, every parameter looks fine except P2_Gain is too low (lower than 8). Is that color balance off is caused by the concatamerization of adaptors? We did not know this trick and did not dilute the adaptors while our ChIP DNA concentration is very low. If the P2_gain is a result of adaptor concatamerization, we can use it to titrate the adaptor dilution based on the concentration of ChIP Seq samples from our customers.
Thanks in advance
Thanks in advance.
Smallcreek
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