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How does new Truseq Stranded Protocol actually work? Is it dUTP based??
I have not done this work. Please advise. thank you very much.
I have not done this work. Please advise. thank you very much.
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Yes it is dUTP based. Also adds actinomycin D to first strand synthesis, presumably to prevent any second strand synthesis during that step.
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Phillip -
Philip, Thank you very much clarifying this. Did you use this in your laboratory? and how happy are you with this?
thank you.Comment
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Have a kit, did not try it yet. Next week, maybe.
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PhillipComment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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