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  • seqseq123
    Junior Member
    • Oct 2010
    • 3

    #1

    How does new Truseq Stranded Protocol actually work? Is it dUTP based??

    How does new Truseq Stranded Protocol actually work? Is it dUTP based??
    I have not done this work. Please advise. thank you very much.
  • pmiguel
    Senior Member
    • Aug 2008
    • 2328

    #2
    Yes it is dUTP based. Also adds actinomycin D to first strand synthesis, presumably to prevent any second strand synthesis during that step.

    --
    Phillip

    Comment

    • seqseq123
      Junior Member
      • Oct 2010
      • 3

      #3
      Philip, Thank you very much clarifying this. Did you use this in your laboratory? and how happy are you with this?
      thank you.

      Comment

      • pmiguel
        Senior Member
        • Aug 2008
        • 2328

        #4
        Have a kit, did not try it yet. Next week, maybe.

        --
        Phillip

        Comment

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