Tweet
How does new Truseq Stranded Protocol actually work? Is it dUTP based??
I have not done this work. Please advise. thank you very much.
I have not done this work. Please advise. thank you very much.
-
-
Yes it is dUTP based. Also adds actinomycin D to first strand synthesis, presumably to prevent any second strand synthesis during that step.
--
Phillip -
Philip, Thank you very much clarifying this. Did you use this in your laboratory? and how happy are you with this?
thank you.Comment
-
Have a kit, did not try it yet. Next week, maybe.
--
PhillipComment
-
While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...06-06-2024, 07:15 AM -
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...05-24-2024, 01:16 PM
Comment