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Millipore Magna ChIP-seq

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  • Millipore Magna ChIP-seq

    Dear community,

    I'm a ChIP-seq newbie and decided to use the promissing Magna ChIP-seq kit for my experiments. I would be very happy to share thoughts and ideas with you in this thread for the correct use of the kit.

    As a start, I would like to ask you a technical question regarding the ChIP-protocol itself:
    Why we need to boil the ChIPed DNA sample after reverse cross-linking? Would the denatured molecules ever find their complementary strand in such a complex mixture of sequences and can they be subjected to library preparation afterwards?

    Many thanks in advance
    1
    I love this kit because it works perfectly with me
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    I like the kit, but I faced some problems
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    It never worked in my hands
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    I never worked with this kit
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    1

  • #2
    They will..... DNA 101.....

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    • #3
      Thanks for the answer...
      But do I really need to do it?

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      • #4
        I've never seen a ChIP protocol where they ask you to boil the DNA to reverse the cross link; 65C 6h or o/n will do

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        • #5
          Nor me, I did it anyway to stick to their protocol...

          Have a nice day

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          • #6
            I have seen it once in the Magnify protocol (invitrogen) but it was changed to a lower temperature in later versions...

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