Dear community,
I'm a ChIP-seq newbie and decided to use the promissing Magna ChIP-seq kit for my experiments. I would be very happy to share thoughts and ideas with you in this thread for the correct use of the kit.
As a start, I would like to ask you a technical question regarding the ChIP-protocol itself:
Why we need to boil the ChIPed DNA sample after reverse cross-linking? Would the denatured molecules ever find their complementary strand in such a complex mixture of sequences and can they be subjected to library preparation afterwards?
Many thanks in advance
I'm a ChIP-seq newbie and decided to use the promissing Magna ChIP-seq kit for my experiments. I would be very happy to share thoughts and ideas with you in this thread for the correct use of the kit.
As a start, I would like to ask you a technical question regarding the ChIP-protocol itself:
Why we need to boil the ChIPed DNA sample after reverse cross-linking? Would the denatured molecules ever find their complementary strand in such a complex mixture of sequences and can they be subjected to library preparation afterwards?
Many thanks in advance
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