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Actually, our interpretation of the strange bioanalyzer traces after Pippen prep (the old one and with EthBr gel cassettes) was that the ethidiumbromide bound to the DNA leads to aberrant migration and reduced staining with the BA dye. After an additional PCR the the products produce the more or less the expected smears on a BA. We found that the Pippen prep fragments are amazingly accurate even for PCR libraries, despite the Y-adapters. We have not tried long 600 bp fragments, though. We are only using 2% cassettes at the moment.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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