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Actually, our interpretation of the strange bioanalyzer traces after Pippen prep (the old one and with EthBr gel cassettes) was that the ethidiumbromide bound to the DNA leads to aberrant migration and reduced staining with the BA dye. After an additional PCR the the products produce the more or less the expected smears on a BA. We found that the Pippen prep fragments are amazingly accurate even for PCR libraries, despite the Y-adapters. We have not tried long 600 bp fragments, though. We are only using 2% cassettes at the moment.
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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