Actually, our interpretation of the strange bioanalyzer traces after Pippen prep (the old one and with EthBr gel cassettes) was that the ethidiumbromide bound to the DNA leads to aberrant migration and reduced staining with the BA dye. After an additional PCR the the products produce the more or less the expected smears on a BA. We found that the Pippen prep fragments are amazingly accurate even for PCR libraries, despite the Y-adapters. We have not tried long 600 bp fragments, though. We are only using 2% cassettes at the moment.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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