Hi all
I was wondering if anyone had any experience of low concentration DNA and if there was a method to improve extraction?
I am currently taking environmental swabs of quite a small area (which can not be made larger) and doing a bead-beat and Qiagen column extraction.
I am getting around 6ng/ul showing on a Nanodrop and nothing coming up on an endpoint 16S PCR
I was wondering how much starting material you might need to be able to feed into the MiSeq?
Thanks for your help!
nikki
I was wondering if anyone had any experience of low concentration DNA and if there was a method to improve extraction?
I am currently taking environmental swabs of quite a small area (which can not be made larger) and doing a bead-beat and Qiagen column extraction.
I am getting around 6ng/ul showing on a Nanodrop and nothing coming up on an endpoint 16S PCR
I was wondering how much starting material you might need to be able to feed into the MiSeq?
Thanks for your help!
nikki
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