PreCR Repair Mix

[djmastermix]

Hi- Has anyone tried using PreCR Repair Mix from NEB for NGS applications? I am going to test it out but want to know if any has experience using it. Do you know why you use it after shearing and before end repair, rather than before shearing? And do you follow the standard or sequential protocol?

Any help you can offer would be much appreciated! Thanks!

[rnaeye]

I would suggest that you call NEB. They have very good customer service. Actually you talk to scientist who developed the reagent.