Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Ampure XP beads cleanup and concentration

    Does anyone know what ratio of Ampure XP beads:sample I must use to remove material from my library that is 150bp and below? My library is in 30ul of 1x TE.

    Also has anyone ever concentrated their final library in a DNA Speedvac/concentrator unit and sequenced it with reason being the library did not produce enough yield but the speedvac step allowed it to. I'm curious if the sequencing of this concentrated library worked out well?

  • #2
    You could try google! 1.0-1.5 ratio should do it.

    Speedvac does not increase yield, just concentration. You can use it but make sure your library is in H2O.

    Comment


    • #3
      Does anyone know what ratio of Ampure XP beads:sample I must use to remove material from my library that is 150bp and below? My library is in 30ul of 1x TE.
      Look like about 0.8X from this: http://core-genomics.blogspot.ca/201...eads-work.html. But do your own test to make sure.

      Also note that what matters is the amount of buffer, not of beads. You can use a stingy amount of beads and bring up the rest with 20% PEG, 2.5M NaCl. KAPA includes this buffer with their library kit so you can put in beads once for the first enzymatic reaction and then keep reusing the same beads with the same samples.

      Also has anyone ever concentrated their final library in a DNA Speedvac/concentrator unit and sequenced it with reason being the library did not produce enough yield but the speedvac step allowed it to.
      I haven't but Microcon columns are very easy and can get it down to 10-20 µL.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Advanced Tools Transforming the Field of Cytogenomics
        by seqadmin


        At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...
        09-26-2023, 06:26 AM
      • seqadmin
        How RNA-Seq is Transforming Cancer Studies
        by seqadmin



        Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...
        09-07-2023, 11:15 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 09-29-2023, 09:38 AM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-27-2023, 06:57 AM
      0 responses
      13 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-26-2023, 07:53 AM
      0 responses
      30 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-25-2023, 07:42 AM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Working...
      X