Header Leaderboard Ad

Collapse

Ampure XP beads cleanup and concentration

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Ampure XP beads cleanup and concentration

    Does anyone know what ratio of Ampure XP beads:sample I must use to remove material from my library that is 150bp and below? My library is in 30ul of 1x TE.

    Also has anyone ever concentrated their final library in a DNA Speedvac/concentrator unit and sequenced it with reason being the library did not produce enough yield but the speedvac step allowed it to. I'm curious if the sequencing of this concentrated library worked out well?

  • #2
    You could try google! 1.0-1.5 ratio should do it.

    Speedvac does not increase yield, just concentration. You can use it but make sure your library is in H2O.

    Comment


    • #3
      Does anyone know what ratio of Ampure XP beads:sample I must use to remove material from my library that is 150bp and below? My library is in 30ul of 1x TE.
      Look like about 0.8X from this: http://core-genomics.blogspot.ca/201...eads-work.html. But do your own test to make sure.

      Also note that what matters is the amount of buffer, not of beads. You can use a stingy amount of beads and bring up the rest with 20% PEG, 2.5M NaCl. KAPA includes this buffer with their library kit so you can put in beads once for the first enzymatic reaction and then keep reusing the same beads with the same samples.

      Also has anyone ever concentrated their final library in a DNA Speedvac/concentrator unit and sequenced it with reason being the library did not produce enough yield but the speedvac step allowed it to.
      I haven't but Microcon columns are very easy and can get it down to 10-20 ┬ÁL.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
        by seqadmin



        Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
        03-21-2023, 01:49 PM
      • seqadmin
        Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
        by seqadmin




        Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
        03-10-2023, 05:31 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-24-2023, 02:45 PM
      0 responses
      16 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2023, 12:26 PM
      0 responses
      17 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-17-2023, 12:32 PM
      0 responses
      17 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-15-2023, 12:42 PM
      0 responses
      24 views
      0 likes
      Last Post seqadmin  
      Working...
      X