Does anyone know what ratio of Ampure XP beads:sample I must use to remove material from my library that is 150bp and below? My library is in 30ul of 1x TE.
Also note that what matters is the amount of buffer, not of beads. You can use a stingy amount of beads and bring up the rest with 20% PEG, 2.5M NaCl. KAPA includes this buffer with their library kit so you can put in beads once for the first enzymatic reaction and then keep reusing the same beads with the same samples.
Also has anyone ever concentrated their final library in a DNA Speedvac/concentrator unit and sequenced it with reason being the library did not produce enough yield but the speedvac step allowed it to.
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