TruSeq RNA master mix substitution

Laur705

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Join Date: Mar 2013

Posts: 1
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#1

TruSeq RNA master mix substitution

08-20-2013, 11:00 AM

Our lab has been making RNA libraries using a TruSeq RNA kit v2. Right now we have enough of all of the kit reagents for our libraries, except for the second strand master mix.

Has anyone tried substituting other second strand enzyme mix in this protocol? Or using a different protocol from the one in the kit to convert RNA to cDNA before proceeding with library production?
Tags: illumina, protocols, rna, truseq
robertami

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Join Date: Jul 2013

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#2

08-30-2013, 06:54 AM

Hi, my experience with the protocol is that is not easy to get it right even following the protocol and using what they suggest..
BW
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TruSeq RNA master mix substitution

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