HS DNA bioanalyzer result of RNA seq libraries

[Rashmi21481]

Hi All, I am preparing libraries using trueseq V2 kit from illumina. Most of my previous samples look ok on bioanalyzer. Recently, I used low input RNA kit from illumina for 6 samples. The libraries look a bit different from my previous preparations. Can anyone please have a look and let me know if I can still submit these libraries?
Thanks,
Rashmi

[zatoichi888]

It looks like that either you overly amplified your library or you didn't do the size selection well enough.

[subxero]

The size selection looks off, I have had over amplification and it does not look like that.

[pmiguel]

How long did you fragment your RNA?
Illumina has a "low input" RNA kit?

Anyway, they will probably sequence fine, except you have quite a bit of adapter dimer (the peak at 120 bp). You probably should do another ampure clean up.

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Phillip