Electropherogram of insect (S2) RNA

[A_Harrington]

Hey Everyone,

I'm trying to make libraries from S2 cells and have a question regarding what the bioanalyzer electropherogram should look like. I know the RIN numbers for insect RNA are usually pretty low due to the dissociation of the 28S rRNA, but I can't seem to find any examples of "good" insect RNA for a visual comparison. I'm using the pico chip, RIN numbers are between 6-7.5, samples are not heat denatured prior to loading. Thanks!

[ZFHans]

Hi,

We once made some RNAseq libraries from Tribolium castaneum. A trace is added as attachment. Our Bioanalyzer got confused by the trace and refused to give a RIN value. We continued with library prep and got good libraries.
Hope this helps,

Hans Jansen