Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • #46
    We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

    Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

    They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

    Comment


    • #47
      Originally posted by biozlzyc View Post
      The bead is overdried and there is no DNA in the supernate.What should I do?
      Use TE with 0.05% Tween20 and set it to shake at 37. You may also need to sonicate it a bit to break clumps, but I would not do it if DNA was large

      Comment


      • #48
        When you say more effective, you mean the range of the DNA size, distribution or recovery?

        Originally posted by cbird View Post
        We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

        Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

        They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

        Comment


        • #49
          good questions, it give me some help, thanks everyone in this question

          Comment


          • #50
            Anyone tried to purify double strand circular DNA by AmPure XP??

            Anyone tried to purify ~1kb double strand circular DNA by AmPure XP?

            Comment

            Latest Articles

            Collapse

            • seqadmin
              Recent Advances in Sequencing Analysis Tools
              by seqadmin


              The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
              05-06-2024, 07:48 AM
            • seqadmin
              Essential Discoveries and Tools in Epitranscriptomics
              by seqadmin




              The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
              04-22-2024, 07:01 AM

            ad_right_rmr

            Collapse

            News

            Collapse

            Topics Statistics Last Post
            Started by seqadmin, 05-14-2024, 07:03 AM
            0 responses
            26 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 05-10-2024, 06:35 AM
            0 responses
            46 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 05-09-2024, 02:46 PM
            0 responses
            59 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 05-07-2024, 06:57 AM
            0 responses
            47 views
            0 likes
            Last Post seqadmin  
            Working...
            X