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  • cbird
    Member
    • May 2012
    • 10
    #46
    We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

    Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

    They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

    Comment

    • yaximik
      Senior Member
      • Apr 2011
      • 199
      #47
      Originally posted by biozlzyc View Post
      The bead is overdried and there is no DNA in the supernate.What should I do?
      Use TE with 0.05% Tween20 and set it to shake at 37. You may also need to sonicate it a bit to break clumps, but I would not do it if DNA was large

      Comment

      • nextgen
        Member
        • Feb 2010
        • 26
        #48
        When you say more effective, you mean the range of the DNA size, distribution or recovery?

        Originally posted by cbird View Post
        We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

        Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

        They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

        Comment

        • ctguhu
          Junior Member
          • Jul 2014
          • 1
          #49
          good questions, it give me some help, thanks everyone in this question

          Comment

          • aaron_tao
            Junior Member
            • Sep 2010
            • 6
            #50
            Anyone tried to purify double strand circular DNA by AmPure XP??

            Anyone tried to purify ~1kb double strand circular DNA by AmPure XP?

            Comment

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