[cbird]

We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

[yaximik]

The bead is overdried and there is no DNA in the supernate.What should I do?

Use TE with 0.05% Tween20 and set it to shake at 37. You may also need to sonicate it a bit to break clumps, but I would not do it if DNA was large

[nextgen]

When you say more effective, you mean the range of the DNA size, distribution or recovery?

We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

[ctguhu]

good questions, it give me some help, thanks everyone in this question

[aaron_tao]

Anyone tried to purify double strand circular DNA by AmPure XP??

Anyone tried to purify ~1kb double strand circular DNA by AmPure XP?