Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 4
  • Filter
  • Time
  • Show
Clear All
new posts
Previous 1 2 3 4 template Next
  • cbird
    Member
    • May 2012
    • 10
    #46
    We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

    Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

    They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

    Comment

    • yaximik
      Senior Member
      • Apr 2011
      • 199
      #47
      Originally posted by biozlzyc View Post
      The bead is overdried and there is no DNA in the supernate.What should I do?
      Use TE with 0.05% Tween20 and set it to shake at 37. You may also need to sonicate it a bit to break clumps, but I would not do it if DNA was large

      Comment

      • nextgen
        Member
        • Feb 2010
        • 26
        #48
        When you say more effective, you mean the range of the DNA size, distribution or recovery?

        Originally posted by cbird View Post
        We've found SPRI Select is much more effective than Ampure XP for size selection also. Anybody know what the difference is? I seriously doubt it's the beads themselves...

        Check out this paper. http://www.plosone.org/article/info%...l.pone.0010029

        They use varying percentages of PEG and 0.9M NaCl, not 2.5M. They are also using MyOne CA Dynabeads

        Comment

        • ctguhu
          Junior Member
          • Jul 2014
          • 1
          #49
          good questions, it give me some help, thanks everyone in this question

          Comment

          • aaron_tao
            Junior Member
            • Sep 2010
            • 6
            #50
            Anyone tried to purify double strand circular DNA by AmPure XP??

            Anyone tried to purify ~1kb double strand circular DNA by AmPure XP?

            Comment

            Previous 1 2 3 4 template Next

            Latest Articles

            Collapse
            • SEQadmin2
              Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
              by SEQadmin2



              Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
              ...
              07-09-2026, 11:10 AM
            • SEQadmin2
              Cancer Drug Resistance: The Lingering Barrier to Rising Survival
              by SEQadmin2



              Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

              There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
              07-08-2026, 05:17 AM
            • GATTACAT
              Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
              by GATTACAT
              Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
              07-01-2026, 11:43 AM
            View All

            ad_right_rmr

            Collapse

            News

            Collapse
            Topics Statistics Last Post
            UC San Diego Bioengineers Map Gene Function in Human Stem Cells by SEQadmin2
            Started by SEQadmin2, 07-13-2026, 10:26 AM
            0 responses
            15 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            07-13-2026, 10:26 AM  
            New Analysis Splits Leukemia Into 16 Epigenomic Subgroups by SEQadmin2
            Started by SEQadmin2, 07-09-2026, 10:04 AM
            0 responses
            29 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            07-09-2026, 10:04 AM  
            Genome-Wide CRISPR Screen Uncovers Unlikely Psoriasis Target by SEQadmin2
            Started by SEQadmin2, 07-08-2026, 10:08 AM
            0 responses
            16 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            07-08-2026, 10:08 AM  
            Engineered Protein Motor Takes Its First Steps Along DNA Track by SEQadmin2
            Started by SEQadmin2, 07-07-2026, 11:05 AM
            0 responses
            33 views
            0 reactions
            		 Last Post SEQadmin2
            by SEQadmin2
            07-07-2026, 11:05 AM  
            View All
            Working...