Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • sslevine
    Junior Member
    • Nov 2013
    • 1

    ABRF Study to compare accuracy and reproducibility of DNA/RNA quantification methods

    This year, the DNA Sequencing Research Group (DSRG) of the ABRF is conducting a multisite study to better understand what methods labs are using to quantify nucleic acids and to discover best practices. Quantifying RNA and DNA samples is one of the most basic methods used in all sequencing and microarray labs. However, beyond the manufacturers’ specifications, there are few standards to tell us how our instruments are performing relative to their optimum behavior, or to each other. We are hoping to learn how prevalent different technologies are, how much their performance varies from location to location, and to learn how well each instrument preforms. As a first step, we have posted an informational survey at https://www.surveymonkey.com/s/YZTDJY5. If you would be interested in participating in the study, we invite you to fill out the survey or to send me a PM.

    The Association of Biomolecular Resource Facilities (ABRF) is dedicated to advancing core and research biotechnology laboratories through research, communication, and education. ABRF members include over 650 scientists representing 360 institutions world wide, including those in industry, government, academic and research institutions. The ABRF is not affiliated with any commercial supplier of instruments, services or supplies.

    Web survey powered by SurveyMonkey.com. Create your own online survey now with SurveyMonkey's expert certified FREE templates.


    Thank you!
    -Stuart Levine & and the rest of the ABRF DSRG

Latest Articles

Collapse

  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 07-02-2026, 11:08 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-30-2026, 05:37 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
55 views
0 reactions
Last Post SEQadmin2  
Working...