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  • Tom Haltern
    Junior Member
    • Feb 2010
    • 7

    #1

    library preparation cost

    Dear all, I'm trying to get a view of a full economics cost for a library prep for the Illumina GA. I certainly know that there is different commercial kits out there but I was wondering what you estimate/calculate for additional purification steps (not included in the kits), for size selection and for overhead? Thanks. Tom
  • peromhc
    Senior Member
    • Sep 2009
    • 108

    #2
    what type of library do you want? DNA, mRNA, etc...

    Comment

    • Tom Haltern
      Junior Member
      • Feb 2010
      • 7

      #3
      I'm talking fragmented DNA only. No CHIP or RNA....

      Comment

      • peromhc
        Senior Member
        • Sep 2009
        • 108

        #4
        it is about $225 per sample for >10 preps, using NEB kit and a few bioanalyzer runs, plus adapters/primers from IDT or wherever..

        Comment

        • upenn_ngs
          Member
          • Sep 2009
          • 70

          #5
          Originally posted by peromhc View Post
          it is about $225 per sample for >10 preps, using NEB kit and a few bioanalyzer runs, plus adapters/primers from IDT or wherever..
          it is possible to develop a sample prep protocol <$65 per sample

          Comment

          • peromhc
            Senior Member
            • Sep 2009
            • 108

            #6
            Originally posted by upenn_ngs View Post
            it is possible to develop a sample prep protocol <$65 per sample
            Using which reagents? At what scale?

            Comment

            • upenn_ngs
              Member
              • Sep 2009
              • 70

              #7
              Originally posted by peromhc View Post
              Using which reagents? At what scale?
              full scale NEBnext enzymes, primers from Operon, agencourt SPRI bead clean-up

              Comment

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